| 毛细管电泳非接触电导检测器的研制 |
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| 引用本文： | 刘晓为,张海峰,邵宪辉,李玲,彭志成.毛细管电泳非接触电导检测器的研制[J].纳米技术与精密工程,2009,7(2):123-126. |
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| 作者姓名： | 刘晓为 张海峰 邵宪辉 李玲 彭志成 |
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| 作者单位： | 1. 哈尔滨工业大学MEMS中心,哈尔滨,150001;微系统与微结构制造教育部重点实验室,哈尔滨,150001
2. 哈尔滨工业大学MEMS中心,哈尔滨,150001 |
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| 摘 要： | 非接触电导检测法已经成为毛细管电泳芯片中一种通用的物质分离检测方法．本文提出了一种微型化的电容耦合非接触电导分析系统，采用新颖的三明治电极结构减小了电极之间的寄生电容．这种电极结构能够有效地减小电极的长度，克服了非接触电导检测中电极易于折断的缺点．当采用电极宽度1mm、电极间距1mm、MES／His缓冲溶液浓度为20mmol／L和激励电压20Vpp、90kHz的条件时，检测器具有最好的分离效果．在最佳的分离效果，实现了1mmol／L的K^＋和Mg^2＋混合无机阳离子的分离检测．
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| 关 键 词： | 毛细管电泳 非接触电导检测法 PMMA 电极结构 无机阳离子 |
Contactless Conductivity Detector for Capillary Electrophoresis Chip |
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| LIU Xiao-wei,ZHANG Hai-feng,SHAO Xian-hui,LI Ling,PENG Zhi-cheng.Contactless Conductivity Detector for Capillary Electrophoresis Chip[J].Nanotechnology and Precision Engineering,2009,7(2):123-126. |
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| Authors: | LIU Xiao-wei ZHANG Hai-feng SHAO Xian-hui LI Ling PENG Zhi-cheng |
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| Affiliation: | 1. MEMS Center;Harbin Institute of Technology;Harbin 150001;China;2. Key Laboratory of Micro-Systems and Micro-Structures Manufacturing;Ministry of Education;China |
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| Abstract: | Contactless conductivity detection has become an accepted detection method in capillary electrophoresis chip for a variety of analytes. In this paper, a miniaturized analytical system with capacitively coupled contactless conductivity detection was presented. Polymethyl methacrylate (PMMA) was used as substrate to substitute for glass material. A novel sandwich electrode structure was used to minimize the stray capacitance of the detector. The construction can decrease the length of the electrode and overco... |
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| Keywords: | PMMA capillary electrophoresis contactless conductivity detection PMMA electrode structure inorganic cation |
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