海藻酸钠明胶协同固定化酵母生产ATP

采用海藻酸钠与明胶协同固定化酵母合成ATP,考察了海藻酸钠、明胶浓度、酵母包埋量、交联剂浓度与交联时间等因素对固定化酵母胶体的机械强度、合成ATP转化率的影响,以及葡萄糖与牛血清蛋白向固定化酵母胶体内扩散的状况。结果表明,利用非稳态传递模型计算得到葡萄糖的有效扩散系数为5.82×10~(10)m~2/s,而BSA(V)不能扩散进入该固定化颗粒。进一步考察反应条件的影响,确定优化的反应条件为固定化酵母400 g/L.蔗糖80 g/L,KH_2PO_416 g/L,K_2HPO_4 92 g/L,MgSO_4 8 g/L,腺20 g/L,在该条件下得转化率平均在90%以上。

Biosynthesis of Adenosine 5'-Triphosphate by Using Sodium Alginate-gelatin Immobilized Yeast Cells

A method for immobilizing beer yeast with sodium alginate and gelatin was presented.The effect of various reaction parameters,such as concentrations of sodium alginate(SA)and gelatin(GT), quantity of embedded of yeast,concentration of glutaraldehyde and crosslinking time on the compression in- tensity and the ATP conversion rate were studied.Diffusivities from the solution into the immobilized parti- cle,such as glucose and bovine serum album in Fraction V(BSA(V))were also investigated according to the established mathematical model.The effective diffusion parameter of glucose is 5.82×10~(10)m~2/s,but no dif- fusion of BSA(V)into the particle was observed.Some factors which may affect the ATP conversion activity were investigated.On the basis of the experimental results which the optimal synthetic condition is as fol- lows:400 g/L yeast,80 g/L sugar,16 g/L K_2HPO_4,92g/L KH_2PO_4,20 g/L AR,8 g/L Mg_2SO_4.Under0 the condition,the conversion rate of ATP can be maintained over 90% on the average.