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The protective effect of monosodium glutamate on survival of Lactobacillus rhamnosus GG and Lactobacillus rhamnosus E-97800 (E800) strains during spray-drying and storage in trehalose-containing powders
Authors:EO Sunny-Roberts  D Knorr
Affiliation:1. Université de Lorraine, LIBio, Laboratoire d''Ingénierie des Biomolécules, 2 av de la Forêt de Haye, TSA 40602, 54518 Vandoeuvre lès Nancy, France;2. KU Leuven, Centre of Microbial and Plant Genetics, Kasteelpark Arenberg 20, Box 2460, B-3001 Leuven, Belgium;3. University of Antwerp, Department of Bioscience Engineering, Groenenborgerlaan 171, B-2020 Antwerp, Belgium;4. Université de Lorraine, LCPME, Laboratoire de Chimie Physique et Microbiologie pour l''Environnement, UMR 7564, 54600 Villers-lès-Nancy, France;1. Suzhou Key Lab of Green Chemical Engineering, School of Chemical and Environmental Engineering, College of Chemistry, Chemical Engineering and Material Science, Soochow University, Suzhou Industrial Park 215123, Jiangsu, China;2. UMR1253 STLO, Agrocampus Ouest, INRA, F-35042 Rennes, France;1. School of Applied Sciences, RMIT University, 124 La Trobe St, Melbourne, Victoria 3001, Australia;2. Department of Chemical Engineering, Monash University, Clayton, Victoria 3800, Australia;3. Chemical Engineering Innovation Laboratory, College of Chemistry, Chemical Engineering and Material Science, Soochow University, Suzhou, Jiangsu Province, China;1. Université de Lorraine, LIBio, Laboratoire d’Ingénierie des Biomolécules, 2, av de la Forêt de Haye, TSA 40602, F-54518, Vand?uvre-lès-Nancy, France;2. University of Queensland, School of Agricultural and Food Science, St. Lucia, Queensland, 4072, Australia;1. Key Laboratory of Tropical Crop Products Processing of Ministry of Agriculture, Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, 524001, China;2. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China
Abstract:The use of trehalose as a means of preserving Lactobacillus rhamnosus GG (LGG) and L. rhamnosus E-97800 (E800) during spray-drying and the effects of incorporated monosodium glutamate (MSG) in the carrier medium on the survival rates during drying and storage were examined. E800 was more resistant to heat than LGG in 20%, w/w, trehalose; the d-values at 65 °C were 14 s and 5.1 s, respectively. An air outlet temperature of 65–70 °C was taken as optimal for the drying process, as the resultant moisture levels in trehalose containing these bacteria were 4.1% (w/w) and 3.79% (w/w) with corresponding viable counts of 3.65 × 108 cfu mL?1 and 1.80 × 109 cfu mL?1, respectively. The presence of MSG increased the final viable counts of LGG and E800 to 3.05 × 109 cfu mL?1 and 1.30 × 109 cfu mL?1, respectively. Survival of LGG and E800 remained constant at a minimum level of ~108 cfu mL?1 during storage at 25 °C in trehalose–MSG medium.
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