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中心组合设计优化野葛糖化工艺
引用本文:褚 冲,姚尚杰,黄 钧,吴重德,周荣清.中心组合设计优化野葛糖化工艺[J].中国酿造,2016,35(9):145.
作者姓名:褚 冲  姚尚杰  黄 钧  吴重德  周荣清
作者单位:1.四川大学轻纺与食品学院,四川成都610065;2.四川大学制革清洁技术国家工程实验室,四川成都610065
摘    要:以野葛为原料,采用葡萄糖当量值(DE值)为评价指标,在单因素试验的基础上,利用中心组合设计试验优化野葛糖化工艺条件并建立回归方程。结果表明,野葛糖化的最佳工艺参数依次为酶解时间220 min、pH 4.2、酶添加量528 U/g、酶解温度58 ℃。在此工艺条件下糖化液DE值可达到(79.93±0.20)%。

关 键 词:野葛  糖化  葡萄糖当量值  中心组合设计  

Optimization ofsaccharification conditions of Pueraria lobata (Willd.) Ohwi with central composite design
CHU Chong,YAO Shangjie,HUANG Jun,WU Chongde,ZHOU Rongqing.Optimization ofsaccharification conditions of Pueraria lobata (Willd.) Ohwi with central composite design[J].China Brewing,2016,35(9):145.
Authors:CHU Chong  YAO Shangjie  HUANG Jun  WU Chongde  ZHOU Rongqing
Affiliation:1.College of Light Industry, Textile & Food, Sichuan University, Chengdu 610065, China;
2.National Engineering Laboratory for Clean Technology of Leather Production, Chengdu 610065, China
Abstract:Using Pueraria lobata (Willd.) Ohwi as raw material, the saccharification process was optimized by single-factor experiment, and based on this, the optimal saccharification conditions and quadratic regression equation were established by central composite design. The optimal saccharification conditions were obtained as follows: enzymolysis time 220 min, pH 4.2, glucoamylase addition 528 U/g, temperature 58 ℃. Under the conditions, the DE value of saccharification mash could achieve (79.93±0.20)%.
Keywords:Pueraria lobata (Willd  ) Ohwi  saccharification  dextrose equivalent value  central composite design  
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