黑曲霉产胺氧化酶培养及诱导条件的优化

黑曲霉可经诱导产生胺氧化酶，该酶具有催化生物胺氧化脱氨生成相应醛、氨气和过氧化氢的特性。黑曲霉胺氧化酶产生分菌体生长和诱导产酶两个阶段。通过单因素试验优化得出黑曲霉产胺氧化酶的菌体生长最佳培养条件：氮源为NaNO3，碳源为麦芽糖，接种量1%，培养时间24 h；诱导产酶的最佳培养条件：诱导氮源为质量浓度60 g/L的正己胺，诱导碳源为葡萄糖，培养时间36 h，培养温度30 ℃，摇床转速160 r/min，初始pH 7.0，胺氧化酶酶活力从诱导前的1 006.5 U/g提升至1 422.4U/g，提高幅度为41.3%。

Optimization of culture and induction condition of amine oxidase production of Aspergillus niger

Amine oxidase can be produce by induced Aspergillus niger, and has the characteristic of catalyzing biogenic amines to their corresponding aldehydes, H2O2 and NH3 . There are two stages during A. niger amine oxidase production, including cell growth and enzyme induction. Single factor experiments were adopted to optimize the liquid fermentation medium and induction conditions for amine oxidase production by A. niger. The optimum fermentation conditions were nitrogen source NaNO3 , carbon source maltose, inoculum 1%, culture time 24 h. The optimum induction conditions were induction nitrogen source 60 g/L normal hexyl amine, carbon source glucose, culture time 36 h, temperature 30 ℃, rotate speed 160 r/min, and initial pH 7.0. The amine oxidase activity increased from 1 006.5 U/g to 1 422.4 U/g after induction, which increased 41.3%.