新疆昆仑雪菊多糖的分离纯化及其结构的初步鉴定

以新疆昆仑雪菊为原料，采用超声波辅助法提取雪菊多糖（KSCP），经脱蛋白、脱色、透析、DEAE-52层析柱和SephadexG-100凝胶层析柱纯化后，获得两个多糖组分（KSCP1和KSCP2）；将分离纯化后的多糖组分经紫外光谱分析法、冻融分析法、SephadexG-100凝胶柱层析法对其纯度进行鉴定；采用凝胶渗透色谱法和气质联用法（GC-MS）对KSCP分子质量范围和单糖组成进行分析。结果表明，KSCP1和KSCP2均为单一组分；KSCP1是由葡萄糖、阿拉伯糖、半乳糖、木糖4种单糖组成，其摩尔比为10.53∶5.02∶4.96∶1，分子质量范围为8 200～8 700 u；KSCP2主要由葡萄糖、阿拉伯糖、半乳糖3种单糖组成，其摩尔比为1∶2.78∶5.07，分子质量范围为6 100～6 500 u。

Separation and purification of polysaccharides from Kunlun snow chrysanthemum in Xinjiang and preliminary identification of its structure

Using Kunlun snow chrysanthemum in Xinjiang as the raw material, snow chrysanthemum polysaccharides (KSCP) were extracted by ultrasonic-assisted method. The extracts were purified by deproteinization, decoloration, dialysis, DEAE-52 chromatography column and SephadexG-100 gel chromatography column, and two polysaccharide components (KSCP1 and KSCP2) were obtained. The purity of polysaccharide components were identified by ultraviolet spectrum analysis, freezing and thawing method and SephadexG-100 gel column chromatography. Meanwhile, the range of KSCP molecular mass and monosaccharide composition were analyzed by gel permeation chromatography(GPC) and GC-MS. The results showed that KSCP1 and KSCP2 were both single composition. KSCP1 was composed of four kinds of monosaccharide including glucose, arabinose, galactose and xylose, the molar ratio was 10.53∶5.02∶4.96∶1, and the range of molecular mass was 8 200-8 700 u. KSCP2 was composed of three kinds of monosaccharide including glucose, arabinose and galactose, the molar ratio was 1∶2.78∶5.07, and the range of molecular mass was 6 100-6 500 u.