山西老陈醋大曲中优良乳酸菌高密度培养条件优化的研究

该研究以山西老陈醋大曲中分离筛选得到的1株优良乳酸菌戊糖片球菌（Pediococcus pentosaceus）为研究对象，以乳酸菌活菌数为评价指标，通过单因素试验设计研究培养条件（温度、起始pH、接种量）对该菌株生长繁殖的影响；并利用响应面法对该菌株进行高密度培养条件的优化。结果表明，优化后的最佳培养条件为：培养温度25 ℃，初始pH值6.67，接种量5.0%。在此条件下，乳酸菌活菌数为2.89×109 CFU/mL。验证试验表明，实际值与理论值基本相符。

High density cultural conditions optimization of excellent lactic acid bacteria from Shanxi mature vinegar Daqu

Using Pediococcus pentosaceus, an excellent lactic acid bacterium isolated from Daqu of Shanxi mature vinegar as research object, and lactic acid bacteria counts as evaluation index, the effects of temperature, initial pH, and inoculum on the viable counts were studied. High density culture condition of P. pentosaceus was optimized by response surface method, and the optimal conditions were as follows: culture temperature 25 ℃, initial pH 6.67 and inoculum 5.0%. Under these conditions, the count of lactic acid bacteria was 2.89×109 CFU/ml. The verification tests results showed that the actual value was in accord with the theoretical value.