| 培养条件对金黄色葡萄球菌-大肠杆菌混合生物被膜的影响 |
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| 引用本文： | 刘晶晶,陈晶瑜. 培养条件对金黄色葡萄球菌-大肠杆菌混合生物被膜的影响[J]. 中国酿造, 2016, 35(7): 20. DOI: 10.11882/j.issn.0254-5071.2016.07.005 |
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| 作者姓名： | 刘晶晶 陈晶瑜 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院，北京100083 |
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| 基金项目： | 国家自然科学基金面上项目（31371716）；北京高等学校青年英才计划项目（YETP0310） |
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| 摘 要： | 以常见食源性致病菌金黄色葡萄球菌和大肠杆菌为研究对象，采用微孔板法对混合生物被膜形成过程中的不同影响因素进行了研究。结果表明：在25 ℃或30 ℃条件下，采用质量分数0.8%的胰酶大豆肉汤（TSB）或0.8%的脑心浸出液（BHI）培养基培养时，生物被膜形成量较高；添加适量的葡萄糖、乳糖、麦芽糖和蔗糖可提高混合生物被膜形成能力；培养基中NaCl质量分数＞8%时，混合生物被膜的生长明显受到抑制。
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| 关 键 词： | 金黄色葡萄球菌 大肠杆菌 双菌种生物被膜 培养条件 |
Effects of culture conditions on the growth of dual-species biofilm formed by Staphylococcus aureus and Escherichia coli |
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| LIU Jingjing,CHEN Jingyu. Effects of culture conditions on the growth of dual-species biofilm formed by Staphylococcus aureus and Escherichia coli[J]. China Brewing, 2016, 35(7): 20. DOI: 10.11882/j.issn.0254-5071.2016.07.005 |
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| Authors: | LIU Jingjing CHEN Jingyu |
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| Affiliation: | College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China |
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| Abstract: | Effects of different factors on the growth of dual-species biofilm, which was formed by the most common foodborne pathogenic bacterial Staphylococcus aureus and Escherichia coli, were studied by microtiter-plate method. The results showed that S. aureus and E. coli formed high quality biofilms at 25 ℃ or 30 ℃ in 0.8% TSB or BHI medium. The addition of glucose, lactose, maltose, and sucrose improved the growth of the dual-species biofilm, respectively. The formation ability of dual-species was significantly inhibited by adding sodium chloride more than 8%. |
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| Keywords: | Staphylococcus aureus Escherichia coli dual-species biofilm culture condition |
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