| 双酶法水解橡子淀粉工艺研究 |
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| 引用本文： | 郝秋娟,赵士豪,李 娜,马同锁,李 敬,曹芳彦,孙 震. 双酶法水解橡子淀粉工艺研究[J]. 中国酿造, 2016, 35(3): 124. DOI: 10.11882/j.issn.0254-5071.2016.03.028 |
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| 作者姓名： | 郝秋娟 赵士豪 李 娜 马同锁 李 敬 曹芳彦 孙 震 |
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| 作者单位： | 河北经贸大学生物科学与工程学院，河北石家庄050061 |
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| 基金项目： | 河北省科技支撑计划项目（14236802D-2）；河北省社会科学基金项目（HB13GL036）；河北经贸大学2014年大学生创新实验项目（201400052） |
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| 摘 要： | 为掌握中温α-淀粉酶和糖化酶联合水解橡子淀粉的工艺条件，该研究在单因素试验的基础上，运用正交试验设计方法对橡子中的淀粉水解工艺进行了研究和优化。结果表明，橡子淀粉最佳液化工艺条件为中温α-淀粉酶添加量30 U/g，液化温度70 ℃，CaCl2添加量0.3%，液化pH 7.5，液化时间120 min，葡萄糖当量（DE）值为27.79%；最佳糖化工艺条件为糖化酶添加量300 U/g，糖化温度50 ℃，糖化pH 4.5，糖化时间120 min，DE值为48.13%。
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| 关 键 词： | 橡子淀粉 水解 中温&alpha -淀粉酶 糖化酶 |
Hydrolysis technology for acorn starch by double enzymes |
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| HAO Qiujuan,ZHAO Shihao,LI Na,MA Tongsuo,LI Jing,CAO Fangyan,SUN Zhen. Hydrolysis technology for acorn starch by double enzymes[J]. China Brewing, 2016, 35(3): 124. DOI: 10.11882/j.issn.0254-5071.2016.03.028 |
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| Authors: | HAO Qiujuan ZHAO Shihao LI Na MA Tongsuo LI Jing CAO Fangyan SUN Zhen |
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| Affiliation: | College of Biological Science and Engineering, Hebei University of Economics and Business, Shijiazhuang 050061, China |
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| Abstract: | In order to perfect hydrolysis of acorn starch with the mesothermal α-amylase and glucoamylase, the hydrolysis conditions were optimized with orthogonal experiment based on single factor tests. The results showed that the optimal liquefaction process conditions were obtained as follows: mesothermal α-amylase addition 30 U/g, liquefaction temperature 70 ℃, pH 7.5, time 120 min, CaCl2 addition 0.3%, the dextrose equivalent (DE) was 27.79%. The optimal saccharification technology conditions were obtained as follows: glucoamylase addition 300 U/g, saccharification temperature 50 ℃, pH 4.5, time 120 min, and DE was 48.13%. |
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| Keywords: | acorn starch hydrolysis mesothermal α-amylase glucoamylase |
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