多菌混合发酵产纤维素酶及生物法预处理秸秆的研究

对实验室分离筛选的3株绿色木霉和6株枯草芽孢杆菌的生长及产酶情况进行了研究，综合确定绿色木霉绿2与芽孢杆菌S3为混合菌中产纤维素酶酶活最高的组合。在此基础上，采用解脂假丝酵母处理高粱秸秆。先将解脂假丝酵母的种子培养液按照3%的接种量接种到发酵产酶培养基中，隔24 h将绿色木霉绿2的孢子悬浮液按照2.67%的接种量接种到发酵产酶培养基中，再隔12 h将芽孢杆菌S3的种子培养液按照5.33%的接种量接种到发酵产酶培养基中，测定出的滤纸酶活（FPA）最高，为389.89 U/mL，比优化前提高了14.30%。

Multi-strains fermentation for cellulose production and straw pretreatment with biological method

The growth and enzyme production conditions of three strains of Trichoderma viride and six strains of Bacillus subtilis screened were studied. The results showed that the combination of T. viride 2 and B. subtilis S3 had the highest cellulose-producing activities. On this basis, sorghum straw was preprocessed by Candida lipolytica. Firstly, the seed culture of C. albicans was inoculated into the fermentation medium with inoculum 3%. Then after 24 h, spore suspension of T. viride 2 was inoculated into the fermentation medium with inoculum 2.67%. Lastly, after 12 h, the seed culture of B. subtilis S3 was inoculated into the fermentation medium with inoculum 5.33%. Under this condition, the FPA activity was highest of 389.89 U/ml, which increased by 14.30% than before optimization.