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| 响应面法优化黄参多糖的提取工艺研究 | |
| 引用本文： | 黄玉龙,宋 珅,杨晓宇,庞中存,张 继. 响应面法优化黄参多糖的提取工艺研究[J]. 中国酿造, 2016, 35(5): 162. DOI: 10.11882/j.issn.0254-5071.2016.05.034 |
| 作者姓名： | 黄玉龙 宋 珅 杨晓宇 庞中存 张 继 |
| 作者单位： | 1.甘肃省农业科学院农产品贮藏加工研究所，甘肃兰州730070；2.西北师范大学生命科学学院，甘肃兰州730070；3.甘肃省特色植物有效成分制品工程技术研究中心，甘肃兰州730070 |
| 基金项目： | 国家科技支撑计划项目（2012BAD36B04）；国家自然科学基金（51273162） |
| 摘 要： | 该试验优化了黄参多糖的提取工艺。通过单因素试验考察超声功率、提取时间、料液比和提取温度对黄参多糖的影响，依据Box-Behnken中心组合设计，采用响应面法优化黄参多糖超声辅助提取参数。结果表明，最佳提取工艺条件为提取时间12 min、超声功率150 W、提取温度70 ℃、料液比1∶40（g∶mL）、提取次数3次；在此条件下黄参多糖得率为13.33%。采用响应面法优化超声辅助黄参多糖的工艺条件稳定可行。 |
| 关 键 词： | 黄参 多糖 提取 响应面试验 |
Optimization of extraction process for polysaccharide from Sphallerocarpus gracilis by response surface methodology |
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| HUANG Yulong,SONG Shen,YANG Xiaoyu,PANG Zhongcun,ZHANG Ji. Optimization of extraction process for polysaccharide from Sphallerocarpus gracilis by response surface methodology[J]. China Brewing, 2016, 35(5): 162. DOI: 10.11882/j.issn.0254-5071.2016.05.034 | |
| Authors: | HUANG Yulong SONG Shen YANG Xiaoyu PANG Zhongcun ZHANG Ji |
| Affiliation: | 1.Agricultural Product Storage and Processing Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 2.College of Life Science, Northwest Normal University, Lanzhou 730070, China; 3.Bioactive Products Engineering Research Center for Gansu Distinctive Plants, Lanzhou 730070, China |
| Abstract: | The Sphallerocarpus gracilis polysaccharide (SGP) extraction process was optimized. The effects of ultrasounic power, extraction time, temperature and material-liquid ratio on SGP extraction yield were investigated by single factor experiments. Then the SGP extraction process was optimized by Box-Behnken central composite design and response surface methodology. The results showed that the optimal extraction conditions were as follous: ultrasonic time 12 min, power 150 W, temperature 70 ℃, material-liquid ratio 1∶40 (g∶ml), extraction times 3. Under the optimum conditions, the SGP extraction yield was 13.33%. The extraction technology of SGP was stable and viable. |
| Keywords: | Sphallerocarpus gracilis polysaccharide extraction response surface methodology |
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