外源蛋白在毕赤酵母中的高效表达策略

毕赤酵母（Pichia pastoris）表达系统是目前应用最为广泛的蛋白表达系统之一，已成功表达了数百种外源蛋白。但不同外源蛋白的表达量差异较大，能够实现工业化生产的蛋白表达体系并不多，如何提高目的蛋白在该系统中的表达量有着重要的理论和现实意义。该文从发酵工艺方面对影响外源蛋白表达的各种因素做了具体阐述，主要包括培养基的选择与优化、发酵过程参数的控制、分泌型蛋白酶的降解抑制以及诱导型毕赤酵母的甲醇流加机制，旨在提高外源蛋白表达量。

High efficiency expression strategy for heterologous protein in Pichia pastoris

The Pichia pastoris expression system is one of the most widely used protein expression systems. Several hundreds heterologous proteins were expressed successfully. It was successfully expressed several hundred kinds of heterologous protein, but few protein expression system can realize industrialized production. How to improve the expression quantity of target protein in the system has important theoretical and practical significance. Various factors influencing the expression of heterologous proteins in fermentation process were discussed specifically from the aspects of fermentation process, including the selection and optimization of culture medium, control of fermentation parameters, degradation inhibition of secreted protease and mechanism of methanol flow in inducible P. pastoris, in order to improve the expression quantity of heterologous protein.