红曲色素发酵分批补料工艺研究

为了提高红曲菌（Monascus purpureus）FM-4000的红色素色价，采用优化种龄和接种量的方法，探索了分批补料发酵基质对红色素色价的影响。结果表明，最佳种龄为6 h二级种子液，接种量10%（V/V），在发酵48 h时补加20 g/L黄豆粉，在发酵84 h时补加0.5 mol/L氨水，在发酵96 h时补加60 g/L米粉。在此工艺条件下，摇瓶发酵红曲菌FM-4000的红色素色价达到135.61 U/mL，相对初始摇瓶红曲菌FM-4000的红色素色价提高了37.25%；在5 L发酵罐中对红曲菌FM-4000进行分批补料发酵培养，红色素色价可达145 U/mL。

Study on fed-batch fermentation process conditions of Monascus pigments

In order to improve the color value of red pigment produced by Monascus purpureus, the inoculum age and inoculum were optimized, and the effect of fed-batch fermentation substrate on the color value of red pigment was researched. Results showed that the optimum conditions were inoculum age 6 h, inoculum 10% (V/V), feeding 20 g/L soybean meal at fermentation 48 h, 0.5 mol/L aqueous ammonia at 84 h and 60 g/L rice flour at 96 h. Under the conditions, the color value of red pigment produced by M. purpureus FM-4000 in shake flask fermentation was up to 135.61 U/ml, which was 37.25% higher than that before optimization. The color value of red pigment was up to 145 U/ml after M. purpureus FM-4000 fed-batch fermentation in 5 L fermentor.