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Nitric oxide (NO) production in mammalian non-tumorigenic epithelial cells of the small intestine and macrophages induced by individual strains of lactobacilli and bifidobacteria
Authors:Nataša Pipenbaher  Peter Lange Moeller  Jan Dolinšek  Mogens Jakobsen  Hana Weingartl  Avrelija Cencič
Affiliation:1. University of Maribor, Faculty of Agriculture, Vrbanska c.30, 2000 Maribor, Slovenia;2. University of Copenhagen, Department of Food Science, Rolighedsvej 30, 1958 Frederiksberg C, Denmark;3. Canadian Food Inspection Agency, National centre for Foreign Animal Disease, 1015, Arlington Street, Winnipeg, Manitoba R3E 3M4, Canada;4. University of Maribor, Medical Faculty, Slomskov trg 15, 2000 Maribor, Slovenia;1. Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, PR China;2. Xiangya Hospital of Central South University, Changsha 410008, PR China;3. The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, PR China;1. Laboratory of Developmental Nutrition, Department of Animal Science, North Carolina State University, Raleigh, NC 27695-7621, United States;2. Mead Johnson Nutrition, Evansville, IN 47721, United States;1. Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, Chendu 611130, Sichuan, PR China;2. Cheng Du Hua Luo Bio-Tech Col., Ltd, Chengdu 610062, Sichuan, PR China;1. Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA;2. Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA;3. Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA;4. Division of Hematology–Oncology, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA;1. Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China;2. College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, China
Abstract:Nitric oxide (NO) affects multiple gastrointestinal functions, including mucosal inflammation and antimicrobial activity. The aim of this study was to screen the ability of probiotic bacteria to stimulate NO production in porcine intestinal epithelial cells and macrophages in the presence and absence of interferon gamma (INF-γ). Production of NO in intestinal epithelium was stimulated by individual strains of lactobacilli without INF-γ priming. While none of the tested bifidobacteria were capable of inducing NO production, most constitutively secreted NO. Most tested strains induced a significant increase in NO production compared with the control cells in the macrophage cell line 3D4/21. Results support the protective role of the individual strains of the genera Lactobacillus and Bifidobacterium and may lead to new approaches for manipulating and regulating immune responses at the mucosal surfaces of the gastrointestinal tract.
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