响应面法优化褐黄孢链霉菌ZM701产纳他霉素发酵培养基

为了提高褐黄孢链霉菌（Streptomyces gilvosporeus）ZM701生产纳他霉素的产量，该文通过单因素试验，研究碳源、氮源及无机盐对菌体生长和纳他霉素产量的影响，采用响应面分析法优化发酵培养基中葡萄糖、豆粕、CaCO3的添加量。结果表明，褐黄孢链霉菌（S. gilvosporeus）ZM701产纳他霉素最佳发酵培养基组成为：葡萄糖52.6 g/L，酵母蛋白胨3.0 g/L，豆粕23.3 g/L，MgSO4·7H2O 2.0 g/L，CaCO3 3.2 g/L。在此条件下，纳他霉素产量达到5.41 g/L，比优化前提高了77.8%。

Optimization of fermentation media for natamycin production by Streptomyces gilvosporeus ZM701 with response surface methodology

In order to increase the yield of natamycin produced by Streptomyces gilvosporeus ZM701, the effects of carbon source, nitrogen source, and inorganic salts on natamycin production were researched by single factor experiments. The addition of glucose, soybean meal, CaCO3 was optimized by response surface methodology. The results showed that the optimum fermentation media constituents for natamycin production from S. gilvosporeus ZM701 were as followed: glucose 52.6 g/L, yeast peptone 3.0 g/L, soybean meal 23.3 g/L, MgSO4·7H2O 2.0 g/L, CaCO3 3.2 g/L. Under the conditions, natamycin yield was up to 5.41 g/L, which was 77.8% higher than that before optimization.