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| 海洋低温超氧化物歧化酶酵母菌筛选与鉴定 | |
| 引用本文： | 金连豆,王晓辉,迟乃玉,张庆芳,石 群,王 强,张旭姣. 海洋低温超氧化物歧化酶酵母菌筛选与鉴定[J]. 中国酿造, 2016, 35(4): 29. DOI: 10.11882/j.issn.0254-5071.2016.04.007 |
| 作者姓名： | 金连豆 王晓辉 迟乃玉 张庆芳 石 群 王 强 张旭姣 |
| 作者单位： | 1.大连大学生命科学与技术学院，辽宁大连116622；2.辽宁省海洋微生物工程技术研究中心，辽宁大连116622 |
| 基金项目： | 国家高技术研究发展计划‘863计划’（2014AA093512） |
| 摘 要： | 通过邻苯三酚自氧化法测定酶活，从渤海海域中筛选出一株高产超氧化物歧化酶（SOD）酵母菌，其SOD酶活达4 185.67 U/g湿菌体。通过形态学、生理生化鉴定，并结合26S rDNA系统发育分析，初步确定该菌株属于海洋胶红酵母（Rhodotorula sp. CD-008）。粗酶液经SDS-PAGE、Native-PAGE、酶活性染色分析，得到酶的分子质量为37.5 ku，推测SOD类型为Cu/Zn-SOD。为研究超氧化物歧化酶在生物体内抗氧化作用以及在医、药、食品等方面的应用奠定了基础。 |
| 关 键 词： | 超氧化物歧化酶 筛选 鉴定 海洋胶红酵母 酶活性染色 |
Screening and identification of a marine cold-adapted SOD-producing yeast |
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| JIN Liandou,WANG Xiaohui,CHI Naiyu,ZHANG Qingfang,SHI Qun,WANG Qiang,ZHANG Xujiao. Screening and identification of a marine cold-adapted SOD-producing yeast[J]. China Brewing, 2016, 35(4): 29. DOI: 10.11882/j.issn.0254-5071.2016.04.007 | |
| Authors: | JIN Liandou WANG Xiaohui CHI Naiyu ZHANG Qingfang SHI Qun WANG Qiang ZHANG Xujiao |
| Affiliation: | 1.School of Life Science and Biotechnology, Dalian University, Dalian 116622, China; 2.Liaoning Marine Microbial Engineering and Technology Center, Dalian 116622, China |
| Abstract: | A high superoxide dismutase (SOD) yield strain was isolated from the Bohai Sea by measuring the enzyme activity in pyrogallol autoxidation, with enzyme activity of 4 185.67 U/g wet cells. After morphological, physiological, biochemical identification and 26S rDNA phylogenetic analysis, the strain was identified as Rhodotorula sp. CD-008. The crude enzyme liquid was analyzed by SDS-PAGE, Native-PAGE, enzyme activity staining, and the SOD was presumed to be Cu/Zn-SOD with molecular mass of 37.5 ku. The experiment made a foundation for the antioxidant effect of SOD in vivo and the application in clinic, medicine, food and other aspects. |
| Keywords: | SOD screening identification Rhodotorula sp. enzyme activity staining |
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