星虫多糖提取工艺优化及其抗氧化作用研究

以星虫多糖提取率为评价指标，考察不同料液比、超声时间、超声温度、超声功率4个因素对超声辅助提取星虫多糖的影响。在单因素试验的基础上，通过响应面优化试验确定了星虫多糖超声辅助提取的最佳工艺条件为料液比1∶25(g∶mL)，超声温度70 ℃，超声时间50 min，超声功率400 W。在此工艺条件下进行验证试验，星虫多糖提取率的平均值为7.01%。体外抗氧化研究结果表明，星虫多糖对DPPH自由基、羟自由基清除率分别为76.31%、56.93%，有一定的抗氧化作用。

Extraction process optimization and antioxidant activity of Sipunculus nudus polysaccharide

Using Sipunculus nudus polysaccharide extraction yield as evaluation index, the effect of solid-liquid ratio, ultrasonic power, temperature and time on S. nudus polysaccharide extraction yield was studied. Based on single factor experiment results, the optimal extraction technology was determined by Box-Behnken experiment as follows: solid-liquid ratio 1∶25 (g∶ml), temperature 70 ℃, time 50 min, and power 400 W. Under these conditions, verification tests results showed that the average extraction yield of S. nudus polysaccharide was 7.01%. In vitro antioxidant activity results showed that the scavenging effect of S. nudus polysaccharide on DPPH and ·OH was 76.31% and 56.93%, respectively. which showed certain antioxidant activity.