Cellular uptake of protein-bound magnetic nanoparticles in pulsed magnetic field |
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Authors: | Lee Chia-Haw Chen Chao-Bin Chung Ting-Hao Lin Yu-Sheng Lee Wen-Chien |
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Affiliation: | Department of Chemical Engineering, Systems Biology and Tissue Engineering Research Center National Chung Cheng University, Chiayi, 621, Taiwan. |
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Abstract: | A method for fast delivery of proteins conjugated to superparamagnetic iron oxide nanoparticles (SPION) into mammalian cells by applying a strong magnetic field in pulses was proposed. Firstly, SPION were prepared from an alkaline solution of divalent and trivalent iron ions and covalently bound with protein through the activation of N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC). After fluorescently labelling, the protein-nanoparticle conjugate was mixed with mammalian cell line and exposed to a pulsed magnetic field for short durations of few milliseconds. Results suggested that superparamagnetic nanoparticles were able to carry proteins into living cells immediately. Cellular internalization of the fluorescently labelled protein-nanoparticle conjugate was proved by the observation of cell fluorescence in a fluorescent microscopy, as well as cell analysis by a flow cytometer. We found that the cellular uptake was accomplished dominantly by the process of bombardment of magnetic nanoparticles. |
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