| 星点设计-效应面法优化纤维素酶提取黄精多糖 |
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| 引用本文： | 李智慧,黄山,於娜,公衍玲. 星点设计-效应面法优化纤维素酶提取黄精多糖[J]. 化学工业与工程, 2011, 28(4): 44-49 |
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| 作者姓名： | 李智慧 黄山 於娜 公衍玲 |
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| 作者单位： | 青岛科技大学化工学院,山东青岛,266042 |
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| 摘 要： | 采用星点设计-效应面优化法探讨纤维素酶提取黄精粗多糖的最适宜工艺条件。在单因素试验基础上选取酶解温度、酶解pH值和加水量为自变量，黄精粗多糖提取率为因变量，对自变量各水平进行二项式拟合，用效应面法选择较适宜工艺条件，并进行预测分析。结果表明，黄精粗多糖最适宜提取工艺为：纤维素酶用量为黄精质量的0.50％，酶解时间120...
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| 关 键 词： | 星点设计 效应面优化法 黄精多糖 纤维素酶 |
Optimal Extraction of Polysaccharides Polygonatum by Cellulase with Central Composite Design-Response Surface Methodology |
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| LI Zhi-hui,HUANG Shan,YU Na,GONG Yan-ling. Optimal Extraction of Polysaccharides Polygonatum by Cellulase with Central Composite Design-Response Surface Methodology[J]. Chemical Industry and Engineering, 2011, 28(4): 44-49 |
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| Authors: | LI Zhi-hui HUANG Shan YU Na GONG Yan-ling |
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| Affiliation: | LI Zhi-hui,HUANG Shan,YU Na,GONG Yan-ling(College of Chemical Engineering,Qingdao University of Science & Technology,Qingdao 266042,China) |
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| Abstract: | Based on central composite design and response surface methodology,the optimal extraction of Polygonatum polysaccharides by cellulase was investigated.Experiment factors and levels were firstly selected by one factor tests.Independent variables were the enzymolysis temperature,the pH of extracting media and the ratio of liquid,and dependent variable was extraction rate of polysaccharides in Polygonatum.Nolinear mathematic models were used to analysis the relationship between independent and dependent variab... |
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| Keywords: | central composite design response surface methodology Polygonatum polysaccharides cellulase |
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