微波萃取侧柏叶中黄酮类化合物的工艺研究

以侧柏叶中黄酮类化合物的收率为指标，根据正交试验优选出各因素的最适宜提取条件，优选出微波法萃取侧柏叶中黄酮类化合物的最适宜工艺。得到最适宜的工艺条件为：φ(乙醇)60%，料液质量比1∶20，微波时间4 min，微波功率385 W。在此工艺条件下进行验证试验，侧柏叶提取物的收率高达5905%。与直接加热提取法相比，微波提取能大大缩短提取时间，降低提取剂用量，并能提高提取物收率。同时对提取物有效成分进行了定性检测，结果表明侧柏叶提取物确实为黄酮类化合物。

Extractive Technique of Flavonoids from Platycladus Orientalis by Microwave Extraction Method

The extraction of flavonoids Platycladus orientalis by microwave was plimized.Taking the yield of flavonoids as index,the best extraction process was investigated by orthogonal experiment.The optimal condition is： ethanol concentration 60%（V）,solid-liquid mass ratio 1 ∶ 20,microwave time 4 min,microwave power 385 W.Certification experiment was done under these conditions,the extraction yield of flavonoids in Platycladus orientalis was 5.905%.Compared with the direct heating extraction,microwave extraction can greatly shorten the extraction time,decrease the extraction dosage,and improve the yield of the extractants.Quality analysis was investigated for the extracted active ingredients and results showed that the extractants were determined to be from Platycladus orientalis.