| 酸性脲酶的固定化研究 |
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| 引用本文： | 李慧娟,曹智琨,高军,余建勇,刘国华.酸性脲酶的固定化研究[J].化学工业与工程,2011,28(4):50-54. |
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| 作者姓名： | 李慧娟 曹智琨 高军 余建勇 刘国华 |
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| 作者单位： | 山东科技大学化学与环境工程学院,山东青岛,266510 |
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| 基金项目： | 山东科技大学“春蕾计划”项目(2008AZZ074) |
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| 摘 要： | 以明胶为包埋材料，戊二醛为交联剂固定化粪产碱菌所产的酸性脲酶。对酸性脲酶的固定化条件(包括明胶含量、戊二醛浓度、吸附时间、交联时间和粗酶液用量)及酶学性质(温度和pH值)进行了研究。结果表明，固定化酶的最适宜条件为：明胶的质量分数15％，戊二醛质量分数0.3％，吸附时间4 h，交联时间20 min，粗酶液用量4 mL。...
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| 关 键 词： | 酸性脲酶 明胶 戊二醛 固定化 |
Immobilization of Acid Urease |
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| LI Hui-juan,CAO Zhi-kun,GAO Jun,YU Jian-yong,LIU Guo-hua.Immobilization of Acid Urease[J].Chemical Industry and Engineering,2011,28(4):50-54. |
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| Authors: | LI Hui-juan CAO Zhi-kun GAO Jun YU Jian-yong LIU Guo-hua |
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| Affiliation: | LI Hui-juan,CAO Zhi-kun,GAO Jun,YU Jian-yong,LIU Guo-hua(College of Chemical and Environmental Engineering,Shandong University of Science and Technology,Qingdao 266510,China) |
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| Abstract: | Acid urease of Alcaligenes faecalis was immobilized onto the gelatin crosslinked with glutaraldehyde.Immobilization conditions and enzymatic properties of acid urease were determined.The optimum conditions of immobilized urease were as follows： gelatin content 15%,glutaraldehyde volume fraction 0.3%,absorption time 4 h,crosslinking time 20 min,crude enzyme volume 4 mL.Enzymatic characterizations of immobilized and free urease showed that the optimum temperature was 50 ℃ and 30 ℃,the optimum pH value was pH 4.0 and pH 5.0,respectively.Compared with free enzyme,the immobilized urease was more fit for dealing with urea waste water. |
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| Keywords: | acid urease gelatin glutaraldehyde immobilization |
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