| 4,9-二[N-(2-二甲氨基)乙基]-9-吖啶胺-4-甲酰胺与蛋白质相互作用 |
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| 引用本文： | 张丽娟.4,9-二[N-(2-二甲氨基)乙基]-9-吖啶胺-4-甲酰胺与蛋白质相互作用[J].化学工业与工程,2011,28(3):45-48. |
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| 作者姓名： | 张丽娟 |
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| 作者单位： | 福建生物工程职业技术学院药学系,福建福州,350002 |
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| 摘 要： | 应用水溶性4,9一二N-(2一二甲氨基)乙基]-9-吖啶胺-4-甲酞胺(DNAF)探针,研究了DNAF与牛血清白蛋白(BSA)的相互结合作用机理.计算出相应的热力学参数△H,△G以及△S;通过荧光猝灭方法研究结合作用,测定出结合位点数n和表观结合常数KA;根据荧光共振能童转移理论求得供体BSA和受体DNAF的距离r;...
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| 关 键 词： | 4 9-二[N-(2-二甲氨基)乙基]-9-吖啶胺-4-甲酰胺 蛋白质 相互作用机理 |
Interaction between 4,9-di[N-(2-dimethylamino)ethyl]-9-Aminoacridine-4-Formamide and Protein |
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| MANG Li-juan.Interaction between 4,9-di[N-(2-dimethylamino)ethyl]-9-Aminoacridine-4-Formamide and Protein[J].Chemical Industry and Engineering,2011,28(3):45-48. |
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| Authors: | MANG Li-juan |
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| Affiliation: | ZHANG Li-juan(Department of Pharmacy,Fujian Vocational and Technical Institute of Biological Engineering,Fuzhou 350002,China) |
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| Abstract: | Mutual combination mechanism of DNAF with bovine serum albumin（BSA） was investigated by applying water-soluble 4,9-diN-（2-dimethylamino） ethyl]-9-aminoacridine-4-formamide（DNAF） probe.The corresponding thermodynamic parameters ΔH,ΔG and ΔS were calculated and through fluorescence quenching method,the number of binding sites n and apparent binding constant KA were determined.The distance of donor BSA and receptor DNAF,namely r was derived based on the theory of fluorescence resonance energy transfer.The quenching mechanism of DNAF on intrinsic fluorescence quenching of protein and the types of interaction forces were proposed. |
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| Keywords: | 4 9-di[N-(2-dimethylamino) ethyl]-9-aminoacridine-4-formamide protein interaction mechanism |
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