Exploring bioaugmentation strategies for azo dye CI Reactive Violet 5 decolourization using bacterial mixture: dye response surface methodology |
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Authors: | Lamia Ayed Karima Bekir Sami Achour Abdelkrim Cheref Amina Bakhrouf |
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Affiliation: | 1. Faculté de Pharmacie, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Laboratoire d'Analyse, Monastir, Tunisie;2. Institut Supérieur de Biotechnologie de Sfax, Sfax, Tunisie;3. Faculté de Sciences de Tunis, Laboratoire de Chimie des Substances Naturelles et de Synthèse Organique (99/UR/12‐26), Tunisie |
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Abstract: | The use of response surface methodology based on statistical design of experiments is becoming increasingly widespread in several sciences such as analytical and environmental chemistry. In the present study, the decolourization and the degradation efficiency of CI Reactive Violet 5 (CIRV5) was studied using a novel bacterial consortium. CIRV5 (1000 p.p.m.) biodegradation was investigated under shaking condition in mineral salt medium solution (MSM) at a 7.5 pH and a 25°C temperature. The degradation pathways were also predicted, using UV‐visible spectroscopy analysis. Under optimal conditions, the bacterial consortium was able to decolourize the dye completely (>99%) within 8 h. The colour and chemical oxygen demand (COD) removal were 99.29 and 94.93%, respectively. Polymerase chain reaction (PCR) technique was utilized to detect the adhesin genes ‘icaA’ and ‘icaD.’ Our results showed that Staphylococcus aureus had a high decolourization capacity. Phytotoxicity study using Triticum turgidum ssp durum showed the no toxicity of the produced products. |
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Keywords: | azo dye CIRV5 ‘ icaA’ and ‘ icaD’ adhesin genes response surface design Staphylococcus aureus toxicity |
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