| 荧光显微镜观察银杏内酯B对大鼠心肌细胞凋亡的影响 |
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| 引用本文： | 于力方,廖杰,王珊,李宁,梅世昌.荧光显微镜观察银杏内酯B对大鼠心肌细胞凋亡的影响[J].现代科学仪器,2007,17(3):70-72. |
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| 作者姓名： | 于力方 廖杰 王珊 李宁 梅世昌 |
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| 作者单位： | 解放军总医院医学实验测试中心,北京,100853 |
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| 摘 要： | 目的：建立一种简便、快速的细胞凋亡检测方法，探讨银杏内酯B（GB）对心肌细胞凋亡的影响。方法：体外培养大鼠心肌细胞，利用荧光显微镜观察经Hoeehst 33258染色的心肌细胞，记录凋亡细胞核形态学改变并计算凋亡百分率。结果：H2O2可诱导心肌细胞发生凋亡，与H2O2组相比，GB组大鼠心肌细胞凋亡率明显降低，并有显著性差异（P〈0．05）。结论：银杏内酯B对大鼠心肌细胞凋亡具有保护作用。
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| 关 键 词： | 细胞凋亡 心肌细胞 银杏内酯B |
| 收稿时间： | 2006/9/10 |
| 修稿时间： | 2006-09-10 |
Effects of Ginkgolide B on the Apoptosis in Rat Cardiomyocytes by Fluorescent Microscope |
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| Yu Lifang,Liao Jie,Wang Shan,Li Ning,Mei Shichang.Effects of Ginkgolide B on the Apoptosis in Rat Cardiomyocytes by Fluorescent Microscope[J].Modern Scientific Instruments,2007,17(3):70-72. |
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| Authors: | Yu Lifang Liao Jie Wang Shan Li Ning Mei Shichang |
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| Affiliation: | Medical Experiment and Analysis Center, PLA General Hospital, Beijing 100853, China |
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| Abstract: | Objective:A method was developed to determine the apoptosis rapidly.We observed the effect of Ginkgolide B(GB)on the apoptosis in rat cardiomyocytes.Methods:Rats cardiomyocytes were cultured in vitro.Apoptotic morphological changes and percentage of apoptosis were assayed with Hoechst 33258 staining and were observed under fluorescent microscope.Results:H2O2 caused apoptosis on the cultured rat cardiomyocytes.After treatment with GB,rat cardiomyocytes apoptosis decreased significantly(P<0.05).Conclusions:GB might exert protective effects on rat cardiomyocytes against apoptosis. |
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| Keywords: | Cell Apoptosis cardiomyocytes ginkgolide B |
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