| 茶树菇凝集素AAL、AAL-2检测小鼠CD8~+T淋巴细胞表面糖基化研究 |
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| 引用本文： | 雷桂炎,张晓敏,文钰棣,巫伟鹏,黄碧霞,关鑫,梁一. 茶树菇凝集素AAL、AAL-2检测小鼠CD8~+T淋巴细胞表面糖基化研究[J]. 现代食品科技, 2017, 33(1): 8-13 |
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| 作者姓名： | 雷桂炎 张晓敏 文钰棣 巫伟鹏 黄碧霞 关鑫 梁一 |
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| 作者单位： | （广东医科大学医学检验学院临床免疫教研室，广东东莞 523808）,（广东医科大学医学检验学院临床免疫教研室，广东东莞 523808）,（广东医科大学医学检验学院临床免疫教研室，广东东莞 523808）,（广东医科大学医学检验学院临床免疫教研室，广东东莞 523808）,（广东医科大学医学检验学院临床免疫教研室，广东东莞 523808）,（广东医科大学医学检验学院临床免疫教研室，广东东莞 523808）,（广东医科大学医学检验学院临床免疫教研室，广东东莞 523808） |
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| 基金项目： | 国家自然科学基金项目（81102850）；广东省卫生厅基金项目（A2011434）；广东省教育厅育苗工程项目（LYM11070）；东莞市科技局项目（2011108102049）；湛江市科技攻关项目（2011C3109015）；广东医学院大学生创新实验项目（2013ZYDC004） |
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| 摘 要： | 本研究使用两种大型真菌茶树菇新型凝集素AAL(Agrocybe aegerita lectin)和AAL2(Agrocybe aegerita lectin 2),以检测小鼠CD8~+T淋巴细胞表面糖基化。用生物素标记的AAL和AAL2结合流式细胞仪,分别分析从C57BL/6小鼠脾脏和腹股沟淋巴结分离出来的CD8~+T淋巴细胞表面的糖基化水平,及其细胞因子表达水平。结果发现脾脏中AAL~+CD8~+,AAL2~+CD8~+T淋巴细胞所占的比例(%)分别为6.72±3.00和12.18±5.28,而腹股沟淋巴结的AAL~+CD8~+,AAL2~+CD8~+T淋巴细胞所占的比例(%)分别为12.18±5.28和10.15±3.46。细胞因子表达水平结果发现小鼠脾脏AAL2~+CD8~+T细胞表达perforin的细胞比例低于AAL2-CD8~+T细胞(p0.05,有统计学意义),腹股沟淋巴结中AAL~+CD8~+T细胞表达perforin的细胞比例则高于AAL-CD8~+T细胞(p0.05,有统计学意义)。该数据为研究小鼠脾脏和腹股沟淋巴结CD8~+T细胞表面糖基化提供了新的数据,也为两种新型凝集素AAL和AAL2的应用提供理论依据。
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| 关 键 词： | 茶树菇凝集素；小鼠CD8+T淋巴细胞；糖基化 |
| 收稿时间： | 2015-06-27 |
Glycosylation of CD8+ T Lymphocytes from C57BL/6 Mice Recognized by Agrocybe aegerita Lectins |
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| LEI Gui-yan,ZHANG Xiao-min,WEN Yu-di,WU Wei-peng,HUANG Bi-xi,GUAN Xin and LIANG Yi. Glycosylation of CD8+ T Lymphocytes from C57BL/6 Mice Recognized by Agrocybe aegerita Lectins[J]. Modern Food Science & Technology, 2017, 33(1): 8-13 |
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| Authors: | LEI Gui-yan ZHANG Xiao-min WEN Yu-di WU Wei-peng HUANG Bi-xi GUAN Xin LIANG Yi |
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| Affiliation: | (Department of Clinical Immunology, Guangdong Medical University, Dongguan 523808, China),(Department of Clinical Immunology, Guangdong Medical University, Dongguan 523808, China),(Department of Clinical Immunology, Guangdong Medical University, Dongguan 523808, China),(Department of Clinical Immunology, Guangdong Medical University, Dongguan 523808, China),(Department of Clinical Immunology, Guangdong Medical University, Dongguan 523808, China),(Department of Clinical Immunology, Guangdong Medical University, Dongguan 523808, China) and (Department of Clinical Immunology, Guangdong Medical University, Dongguan 523808, China) |
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| Abstract: | Two novel lectins, AAL (Agrocybe aegerita lectin) and AAL2 (Agrocybe aegerita lectin 2), were employed to detect the glycosylation of CD8+ T lymphocytes from C57BL/6 mouse spleen and inguinal lymph nodes. Biotinylated AAL and AAL2, combined with flow cytometry, were used to analyze the glycosylation of CD8+ T lymphocytes and cytokine expression. The proportion (%) of AAL+CD8+ and AAL2+CD8+ T lymphocytes in spleen was found to be 6.72±3.00 and 12.18±5.28, respectively. The proportion (%) of AAL+CD8+ and AAL2+CD8+ T lymphocytes in lymph nodes was 12.18±5.28 and 10.15±3.46, respectively. Moreover, the frequency of perforin+ cells in spleen AAL2+CD8+ T cells was lower than that in AAL2-CD8+ T cells (p<0.05), and the frequency of perforin+ cells in lymph node AAL+CD8+ T cells was higher than that in AAL-CD8+ T cells (p<0.05). This study provides new data on the glycosylation of spleen and inguinal lymph node CD8+ T cells and the application of two new lectins, AAL and AAL2. |
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| Keywords: | Agrocybe aegerita lectins CD8+ T lymphocytes glycosylation |
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