| 酶法合成L－天冬氨酸的动力学初探 |
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| 引用本文： | 朱建良,徐虹,候钧,王雪根.酶法合成L－天冬氨酸的动力学初探[J].陶瓷科学与艺术,1995(Z1). |
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| 作者姓名： | 朱建良 徐虹 候钧 王雪根 |
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| 作者单位： | 南京化工大学生物工程与科学系 |
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| 摘 要： | 进行了由富马酸转氨生成Ｌ－天冬氨酸的酶反应动力学的初步研究，考察了酶活性的稳定性、底物浓度和酶浓度对酶反应速率的影响．针对高浓度底物对酶反应的抑制作用，提出了一个动力学模型方程：ｒ＝２１．４４ｅ０／（１＋０．０１０４４／Ｓ＋Ｓ／２．６３）－８９．７（Ｓ—０．０６４）２该模型方程在ｓ＝０．０２～０．１２ｋｇ／Ｌ范围内能较好地拟合实验数据。
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| 关 键 词： | L－天冬氨酸 动力学 酶反应 |
KINETIC STUDIES OF ENZYME REACTION OF L-ASPARTIC ACID FROM FUMARIC ACID |
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| Zhu Jianliang, Xu Hong, Hou Jun, Wang Xuegen.KINETIC STUDIES OF ENZYME REACTION OF L-ASPARTIC ACID FROM FUMARIC ACID[J].Ceramics Science & Art,1995(Z1). |
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| Authors: | Zhu Jianliang Xu Hong Hou Jun Wang Xuegen |
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| Abstract: | The kinetics of the enzyme reaction, which converts fumaric acid into L--aspartic acid, has been studied. For the inhibitioh of the substrate in high concentration to the reaction, a model was derived from the reaction mechanism, which can be expressed as:Tg =21.44e0/(1 0.01044/S S/2.63)-89.7(S-0.064)2The results calculated by this model were in good agreement with experimental values at S=0.02-0.12g/mL |
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| Keywords: | L-aspartic acid kinetics enzyme reaction |
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