| 类球红细菌突变株及其应用 |
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| 引用本文： | 党磊,靳明慧,田菁,王玮,王小雪,庞欣. 类球红细菌突变株及其应用[J]. 生命科学仪器, 2010, 8(6): 53-56 |
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| 作者姓名： | 党磊 靳明慧 田菁 王玮 王小雪 庞欣 |
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| 作者单位： | 中国空间技术研究院空间生物实验室,北京100190 |
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| 摘 要： | 本研究以类球红细菌1.1737为出发菌株,经空间搭载实验,从回收样品中筛选得到了一株颜色变浅的类球红细菌突变株,利用高效液相色谱分析发现该突变株的辅酶Q10产量较出发菌株提高了30.4%。另外柱层析提纯的结果显示突变株发酵液的分离效率优于出发菌株。
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| 关 键 词： | 空间诱变 类球红细菌 辅酶Q10 柱层析 |
Research and Application of Mutant Rhodobacter sphaeroides |
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| DANG Lei,JIN Ming-hui,TIAN Jing,WANG Wei,WANG Xiao-xue,PANG Xin. Research and Application of Mutant Rhodobacter sphaeroides[J]. Life Science Instruments, 2010, 8(6): 53-56 |
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| Authors: | DANG Lei JIN Ming-hui TIAN Jing WANG Wei WANG Xiao-xue PANG Xin |
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| Affiliation: | (Space Biology R＆D Center,China Academy of Space Technology(CAST)) |
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| Abstract: | In this experiment we screened a high-yielding mutant of Rhodobacter sphaeroides 1.1737 in CoQ10 production with spaceflight. Compared to the control,The mutant colony appeared as white,different from their original red color. The CoQ10 production of mutant strain was 30.4% higher than control. The result of column chromatography demonstrated that the CoQ10 extraction of the mutant stain was easier than Rhodobacter sphaeroides 1.1737. |
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| Keywords: | spaceflight mutation Rhodobacter sphaeroides CoQ10 column chromatography. |
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