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Development of a microconductometric biosniffer for detection of trimethylamine |
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| Authors: | Chrystelle Fillit Nicole Jaffrezic-Renault Francois Bessueille Didier Leonard Kohji Mitsubayashi Jacques Tardy |
| Affiliation: | aLaboratory of Analytical Sciences (UMR CNRS 5180) Claude Bernard University Lyon 1, 69622 Villeurbanne Cedex, France bDepartment of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan cINL (UMR CNRS 5270) Ecole Centrale de Lyon, 69134 Ecully Cedex, France |
| Abstract: | The paper describes the development of a conductometric biosensor for detecting trimethylamine (TMA) which is a good parameter for estimating fish freshness. The biosensor consists of two parts: a microconductometric transducer and a structured enzymatic membrane using a flavin-containing monooxygenase 3 (FMO3) which is known for its sensitivity and selectivity for TMA. The process parameters for the fabrication of the biosniffer and various experimental variables such as pH, time of exposure to glutaraldehyde vapour were investigated with regard to their influence on sensitivity and limit of detection. A low detection limit of 2 μg/mL (ppm) and a linear range up to 40 μg/mL (ppm) were obtained. 90% response was reached in less than 10 min. |
| Keywords: | Conductometric biosensor Trimethylamine Flavin-containing monooxygenase 3 |
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