海带岩藻多糖的水提制备及其抗氧化活性研究

以热水浸提后乙醇沉淀的方法从海带中提取制备岩藻多糖。通过单因素试验和正交优化试验确定最佳提取工艺，可获得岩藻多糖最大提取率为4.99%，硫酸- 苯酚法测定多糖的含量为96.9%。多糖提取物对羟自由基、超氧阴离子自由基的清除作用，还原力测定以及在模拟胃液条件下对NO2 的清除实验结果表明，海带中岩藻多糖提取物具有较强的体外抗氧化性能，且随着多糖质量浓度的增大，其抗氧化活性整体呈逐渐增强趋势。

Water Extraction and Antioxidant Activity of Fucoidan from Laminaria japonica

Hot water extraction followed by ethanol precipitation was used for production of fucoidan from Laminaria japonica in this study. To maximize fucoidan yield, the optimal values of crucial parameters of hot water extraction, including extraction temperature and time and material/liquid ratio were determined by single factor and orthogonal array methods to be 70 ℃, 6 h and 1:40, respectively. Under the above conditions, the maximum yield of fucoidan with 96.9% purity was up to 4.99%. Laminaria japonica fucoidan had strong antioxidant performance in vitro, which was reflected by hydroxyl and superoxide anion free radical scavenging activities, reducing power and nitrite scavenging activity under simulated gastric environment, and its antioxidant performance in vitro exhibited a gradual increase with its increasing concentration.