ELISA detection of IL-1 beta in human sera needs independent confirmation. False positives in hospitalized patients |
| |
Authors: | DJ Herzyk MD Wewers |
| |
Affiliation: | Pharmacology Department, A. Menarini Pharmaceuticals, Florence, Italy. |
| |
Abstract: | Electrical field stimulation evoked a reproducible outflow of calcitonin gene-related peptide-like immunoreactivity (CGRP-LI) from the dorsal half of the rat spinal cord, an effect which was abolished by prior application of capsaicin, tetrodotoxin or removal of extracellular Ca2+. Adenosine (EC50 3.2 microM) and the selective adenosine A1 receptor agonist N6-cyclohexyladenosine (EC50 8.2 nM) inhibited evoked CGRP-LI outflow, while the selective adenosine A2 receptor agonist CGS-21680 was ineffective up to 10 microM. The action of adenosine was prevented by the adenosine A1 receptor selective antagonist 8-cyclopentyl-1,3-dipropylxanthine (30 microM), which did not affect CGRP-LI release on its own. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|