| 大肠杆菌DH12S菌株高效电击转化条件的探讨(英文) |
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| 引用本文： | 陈立,齐香君.大肠杆菌DH12S菌株高效电击转化条件的探讨(英文)[J].西北轻工业学院学报,2010,28(1):30-32,36. |
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| 作者姓名： | 陈立 齐香君 |
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| 作者单位： | 陕西科技大学生命科学与工程学院,陕西西安710021 |
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| 摘 要： | 探讨了电击转化法介导质粒DNA转化到大肠杆菌DH12S的最佳条件，以提高其转化效率．将pUC19质粒转化大肠杆菌DH12S菌株，观察不同的电击转化条件对转化效率的影响．结果表明，电击缓冲液的离子强度、细菌生长状态、感受态细胞保存时间及质粒DNA浓度对转化效率均有不同程度的影响；在电压2．5kV，电阻200Ω，电容25μF，脉冲时间4．3ms和低离子强度电击缓冲液的条件下能获得较高的电击转化率，证明该优化电击转化条件能提高转化效率．
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| 关 键 词： | 电击转化 质粒pUC19 大肠杆菌DH12S |
ELECTROPORATION CONDITIONS OF HIGH TRANSFORMATION EFFICIENCY OF E. COLI DH12S |
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| Abstract: | This paper explored the optimal protocol of transformation of plasmid DNA into Escherichia coli(E.coli) DH12S mediated by electroporation and improved its transformation efficiency,and transferred plasmid pUC19 into E.coli DH12S strain by electroporation using different electrotransformation conditions and observed transformation efficiency.The transformation efficiency was affected by ionic strength of electroshock buffer,growth phase of germ,storage duration of competent cells and concentration of plasmid... |
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| Keywords: | electroporation transformation plasmid pUC19 E coli DH12S |
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