| L-乳酸菌的选育及在酒糟基质中的培养 |
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| 引用本文： | 石孔泉,石贵阳,张梁,章克昌. L-乳酸菌的选育及在酒糟基质中的培养[J]. 酿酒科技, 2006, 0(2): 17-20 |
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| 作者姓名： | 石孔泉 石贵阳 张梁 章克昌 |
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| 作者单位： | 江南大学生物工程学院生物资源研究室;江南大学生物工程学院生物资源研究室;江南大学工业生物技术教育部重点实验室,江苏,无锡,214036 |
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| 摘 要： | 从市售酸乳、泡菜中筛选得到产L-乳酸细菌，通过NTG诱变选育和摇瓶发酵试验，选出两株利用葡萄糖较好的菌株m-58和m-16，以玉米浓醪酒糟清液为基质对2株乳酸细菌进一步驯化培养，得出m-58菌株，更能较好地发酵玉米酒：糟清液，产生乳酸，m-58菌株在48h发酵含糖63gm的玉米浓醪酒糟清液产生50gm的L-乳酸，m-58菌株适合用于发酵玉米酒糟产乳酸。
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| 关 键 词： | 微生物 L-乳酸 筛选 玉米浓醪酒糟 |
| 文章编号： | 1001-9286(2006)02-0017-04 |
| 收稿时间： | 2005-10-26 |
| 修稿时间： | 2005-10-26 |
Screening of L-lactic acid-producing Strain and Its Prime Culture in the Substrate of Distiller's Grains |
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| SHI Kong-quan,SHI Gui-yang,ZHANG Liang,ZHANG Ke-chang. Screening of L-lactic acid-producing Strain and Its Prime Culture in the Substrate of Distiller's Grains[J]. Liquor-making Science & Technology, 2006, 0(2): 17-20 |
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| Authors: | SHI Kong-quan SHI Gui-yang ZHANG Liang ZHANG Ke-chang |
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| Affiliation: | 1. Institute of Biomass Resources, School of Biotechnology, Southern Yangtze University; 2 The Key Lab of Industry Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi, Jiangsu 214036, China |
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| Abstract: | L-lactic acid-producing bacteria were screened from sour milk and marinated vegetables,and after times of NTG mutation screening and flask shaking fermentation tests,two strains including m-58 strain and m-16 strain were obtained,then after further domesticated cultivation of both the two strains in the clear solution of distiller's grains of very high gravity(VHG)ethanol fermentation from corn,m-58 strain was thought to be a good strain for its efficient utilization of the substrate of VHG ethanol fermentation from corn,which could produce 50 g/L L-lactic acid by fermenting 63 g/L corn high gravity mash distiller's grains within 48 h.(Tran.by YUE Yang) |
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| Keywords: | microbe L-lactic acid screening distiller's grains of corn thick mash |
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