藤茶类产品中功效成分二氢杨梅素超高效液相色谱检测方法研究

本研究针对藤茶类保健食品建立了功效成分二氢杨梅素的超高效液相色谱-紫外检测方法.对提取条件进行了优化,确定了提取方式:以藤茶植物形式制作的保健食品采用65%乙醇加热回流提取、以藤茶粗提物配制的保健食品采用65%乙醇超声提取功效成分二氢杨梅素.对色谱条件进行了优化,确定了色谱条件:色谱柱为Acquity UPLCTM B...

Study on determination of dihydromyricetin in Ampelopsis grossedentata by UPLC

In this study,a method for determination of dihydromyricetin,efficiency ingredient of health foodsAmpelopsis grossedentata,was established by UPLC-UV.The optimized extraction conditons were listed： the health foods produced from the plant were extracted with ethanol（65%） under the heat reflux extraction,while the health foods dispensed from the crude extracts were extracted with ethanol（65%） under the ultrasound extraction.The optimized chromatographic conditions were as follows： the seperation column was Acquity UPLCTM BEH C18 100 mm×2.1 mm（i.d）,1.7μm;the mobile phase was acetrile-2% acetic acid solution（15＋85 V＋V） with the flow rate of 0.3 mL/min;the contents of acetrile in the mobile phase were 15% hold for the first 2 min,increased linearly to 20% within 1 min,then increased linearly to 90% within 4 min and hold for 1 min,finally decrease linearly to 15% within 3 min and hold for 6 min;the detector waverlength was 294nm;the column temperature was25℃.The assessment results of the analytical method were showed： the standard solution of dihydromyricetin showed good linear in 2.0 μg/mL~200.0 μg/mL,the average recoveries were 89.6%~96.1%,the RSD were 4.1%~8.0%（n=5）,the LOD was 2.0ng.the analytical method showed satisfsctory results when applied in real samples.