| L-半胱氨酸修饰的Au／Fe_3O_4磁性复合粒子的制备及其对牛血清蛋白的吸附性能 |
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| 引用本文： | 陈志军,汤凯,杨清香,王雪兆,朱海燕,魏永豪. L-半胱氨酸修饰的Au／Fe_3O_4磁性复合粒子的制备及其对牛血清蛋白的吸附性能[J]. 郑州轻工业学院学报(自然科学版), 2013, 0(6): 39-43 |
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| 作者姓名： | 陈志军 汤凯 杨清香 王雪兆 朱海燕 魏永豪 |
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| 作者单位： | 郑州轻工业学院材料与化学工程学院,河南郑州450001 |
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| 基金项目： | 国家自然科学基金项目（20976168,21271160）;河南省高校科技创新人才支持计划项目（2008HASTIT019） |
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| 摘 要： | 采用L-半胱氨酸在Fe_3O_4表面键接Au纳米粒子的方法，制备了L-半胱氨酸修饰的Au／Fe_3O_4磁性复合粒子（LC—Au／Fe_3O_4），并将其用于吸附牛血清蛋白（BSA）．通过紫外可见分光光度计、x-射线衍射仪、傅里叶红外光谱仪、扫描电镜、透射电子显微镜、Zeta电位仪对样品的光学性质、结构形貌、稳定性进行表征，结果表明：复合粒子中的Fe_3O_4具有尖晶石结构，粒径在200nm左右，稳定性较好．对BSA的吸附实验结果表明：LC—Au／Fe304的BSA单位吸附量达到161．5mg／g．
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| 关 键 词： | Au Fe304磁性复合粒子 L-半胱氨酸 牛血清蛋白吸附 |
Preparation of L-cysteine modified Au/Fe_3O_4magnetic composite particle and its adsorption for bovine serum albumin |
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| CHEN Zhi-jun,TANG Kai,YANG Qing-xiang,WANG Xue-zhao,ZHU Hai-yan,WEI Yong-hao. Preparation of L-cysteine modified Au/Fe_3O_4magnetic composite particle and its adsorption for bovine serum albumin[J]. Journal of Zhengzhou Institute of Light Industry(Natural Science), 2013, 0(6): 39-43 |
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| Authors: | CHEN Zhi-jun TANG Kai YANG Qing-xiang WANG Xue-zhao ZHU Hai-yan WEI Yong-hao |
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| Affiliation: | ( College of Material and Chemical Engineering ,Zhengzhou University of Light Industry ,Zhengzhou 450001, China) |
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| Abstract: | The L-cysteine modified Au/Fe_3O_4 magnetic composite particles （LC-Au/Fe_3O_4 ） were synthe- sized and their adsorption properties for bovine serum albumin （BSA） were investigated. The obtained sam- ples were characterized by UV-Vis spectrophotometer（UV-vis） ,X-ray diffraction（ XRD）, Fourier transform infrared spectroscopy （ FT-IR）, scanning electron microscope （ SEM ）, transmission electron microscope （TEM） and Zeta potential, respectively. The results showed that Fe_3O_4 of LC-Au/Fe_3O_4 has a spinel struc- ture ; its diameter was about 200 nm, and the particles showed good stability. The adsorption capacity of LC- Au/Fe_3O_4 composite particles for BSA was 161.5 mg/g. |
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| Keywords: | Au/Fe_3O_4 magnetic composite particle L-cysteine bovine serum albumin adsorption |
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