低次烟叶中的类胡萝卜素降解研究

分别利用生物法和物理法处理低次烟叶，降解烟叶中的类胡萝卜素．生物法是从烟草土壤中分离得到能够有效降解β-胡萝卜素的丝孢酵母．GC—Ms分析表明，该酵母可以彻底降解卢一胡萝卜素．物理法是用臭氧处理低次烟叶，GC—MS分析发现烟叶中的类胡萝卜素和叶绿素a含量随处理时间延长先下降后趋平稳；而且臭氧处理后，烟叶中尼古丁、新植二烯、碳十八酸的含量有所减少，醇类、酯类和酮类化合物在总量上均有提升，可起到醇和烟香、减轻刺激的作用．

Study on degradation of carotenoids in discarded tobacco leaves

Degradation of carotenoids in discarded tobacco leaves by biological and physical treatments was investigated. Biological treatment was to isolate a strain to effectively degrade β-carotene and this strain was identified a yeast Trichosporon. GC-MS analysis showed that this yeast could completely degradeβ-caro- tene. The physical method was to treat discarded tobacco leaves by ozone. GC-MS analysis showed that the contents of carotenoid and chlorophyll increased during the processing time firstly, then decreased and be- came stable. Moreover, after ozone treatment, the contents of alcohols, esters and ketones in tobacco were raised, and the content of nicotine, neophytadiene and carbon stearic acid were reduced to mellow tobacco aroma and reduce irritancy.