Abstract: | In an infusion mash system the concentration of all α amino acids in sweet wort reaches a maximum value when approximately one-third of the total volume extracted has been run from the mashing vessel. The relative concentrations of each α amino acid in wort vary throughout the mash tun extraction owing to differences in rate of extraction of the amino acids and to the quantities produced by peptidase activity in the mash tun. The relative proportions of proline, alanine, glutamine and asparagine in the total α amino nitrogen content of wort decrease appreciably as the mashing process proceeds. Copper boiling results in the partial destruction of methionine, glutamic acid, lysine, arginine and valine present in sweet wort together with an increase in the concentration of ammonium ion in the wort. |