Findings in revision operations for failures after cholesteatoma surgery

A specific, sensitive and simple ELISA for the measurement of human serum apo A II has been developed. The monospecific antibody was raised in goats. The polytyrene plates coated with purified anti-apo A II goat gamma-globulin together with enzyme labelled goat antibodies against human apo A II conjugate were used in this assay. The conjugate was obtained by binding horseradish perioidate by a simplified periodase method. No cross-reactivity with human apo A I, B100, C I, C II, C III and albumin was observed. The minimum measurable concentration of apo A II was 500ng in each assay. A standard curve with a working range of 0.25-8.0 mg/dl was plotted. The coefficients of variation of the reproducibility of intra- and interassays of apo A II in samples were 5.0-8.6% and 6.8-9.9% respectively. The recovery were 106.0 +/- 2.1% (n = 4). The mean concentrations of apo A II in 41 healthy subjects were 24.4 +/- 5.9mg/dl by our method and 26.7 +/- 4.6 mg/dl by RID method, respectively (r = 0.8000, P < 0.001).