Betaine improves the PCR amplification of GC-rich DNA sequences |
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| Authors: | W Henke K Herdel K Jung D Schnorr SA Loening |
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| Affiliation: | Research Division of the Department of Urology, University Hospital Charité, Humboldt University, D-10098 Berlin, Germany. henke@rz.charite.hu-berlin.de |
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| Abstract: | Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondary structure caused by GC-rich regions and, therefore, may be generally applicable to ameliorate the amplification of GC-rich DNA sequences. |
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