富硒灵芝中高抗氧化活力、高硒含量的水溶性硒蛋白的纯化

在富硒灵芝蛋白的各种溶剂提取物中,水溶性蛋白是其中结合硒含量最高的。本文以高硒(Se)含量、高自由基清除能力为考查指标,通过优化的柱层析等方法对富硒灵芝中的水溶性硒蛋白进行了提取、分离和纯化。通过电感偶合等离子体发射光谱法(ICP-AES)测定蛋白质结合的硒元素(se)含量,采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法(SDS-PAGE)进行纯度鉴定。通过电子自旋共振捕捉(EPR)技术测定样品对羟自由基和超氧自由基的清除能力。研究结果表明,纯化后的硒蛋白显示出很强的羟自由基和超氧自由基的清除能力,并且这种抗氧化能力随着硒蛋白中硒含量的增加而增强,从而反映了硒元素在富硒灵芝蛋白抗氧化活力提高方面的重要作用。

Isolation of Water Soluble Selenoprotein with High Se Content and High Antioxidant Activity from Se-enriched Ganoderma Lucidum

Water-soluble protein components extracted from Se-enriched Ganoderma lucidum (Se-GL) was found to contain the highest amount of Se compared withany other reagent-extracted proteins from Se-GL.A kind of seleno-protein,which has relatively higher selenium content and antioxidant activity,was obtained from water-soluble protein components by using optimized chromatography programs.Selenium content was determined by ICP-AES and antioxidant activity was determined by electron paramagnetie resonance (EPR) in conjunction with spin trapping techniques.The results showed that the puritied selno-protein had superoxide and hydroxyl rad- ical scavenging activity and its activity increased with the increase of Se content.This indicated that Se plays an important role in increasing the antioxidant activities of the protein extracts.