黄芩中抗菌有效成分提取工艺研究

在单因素试验的基础上,选取提取温度、提取时间和液料比为影响因素,应用 Box-Behnken中心组合进行3因素3水平的试验设计,以对白色念珠菌产生的抑菌圈直径作为响应值,对结果进行多元线性和二项式拟合,采用响应面(RSA)法选取黄芩中抗菌有效成分提取的最佳工艺条件。最佳工艺条件为:提取温度61 ℃,提取时间2 h,液料比13:1(mL:g),经3次验证实验得黄芩水提物质量浓度为0.5 g/mL时,抑菌圈平均直径为12.637 mm,与预测值12.665 mm接近,阳性对照枯草杆菌肽的平均抑菌圈直径为11.85 mm,表明响应面法优化得到的黄岑水提物对白色念珠菌的抑菌作用显著。

Extraction of Effective Ingredients from Scutellaria Bacalensis Georgi

In order to optimize the extraction condition for effective active ingredient from Scutellaria Bacalensis Georgi, a three-factor-three-level experiments design was developed by using Box-Benhken central composite design method based on single-factor experiments with extraction temperature, extraction time, solvent-solid ratio as influence factors. The bacteriostatic ring diameter against Candida albicans was used as the responsive values. The data were fitted by using multi-linear equation and second-order polynomial equation. And the extraction conditions were optimized by response surface method. The optimum conditions were as follows: extraction temperature 61 ℃, extraction time 2 h and solvent-solid ratio 13:1. Under these conditions,the average diameter of bacteriostatic circles verified by three experiments was 12.637 mm closing to the predicted value(12.664 88 mm),when the concentration of extractions was 0.5 g/mL.And the average diameter of bacteriostatic circle of positive contrast was 11.85 mm.The results showed that the aqueous extract of Scutellaria bacalensis Georgi had significant bacteriostatic effects on Candida albicans.