糠醛渣直接同步糖化发酵生产乙醇过程比较研究

以糠醛渣为原料,直接同步糖化发酵(SSF)生产乙醇,并与水洗糠醛渣生产乙醇进行对比。通过考察不同条件来优化同步糖化发酵生产工艺条件,并分析表征了SSF过程中乙醇浓度和副产物浓度变化。优化条件为:糠醛渣底物质量分数10%,纤维素酶用量12%,无患子皂素质量浓度0.5g/L,酵母接种量7g/L,同步糖化发酵乙醇得率达到其理论得率的93.1%。与水洗糠醛渣相比,糠醛渣直接SSF过程可将原料吸附的5.50%葡萄糖部分转化为乙醇。水洗糠醛渣SSF生产乙醇所产生的副产物要远低于糠醛渣直接生产所产生的副产物,添加无患子皂素可有效抑制糠醛渣同步糖化发酵过程中副产物的产生。

Comparative Study on Conversion Process of Furfural Residue for Ethanol Production by Simultaneous Saccharification and Fermentation

Furfural residues and washed furfural residues were fermented to produce ethanol by simultaneous saccharification and fermentation(SSF). The combination factors(substrate mass fraction, dosage of cellulase, yeast and Sapindus saponin) which affected the concentrations of ethanol and byproduct were optimized by the experiment. The yield of ethanol could reach 93.1% of the theoretical yield during SSF under the conditions of 10% substrate concentration of unwashed furfural residue, 12% cellulase, 0.5g/L Sapindus saponin and 7g/L inoculum solution. The glucose of furfural residues(5.5%) were partly converted to ethanol compared with washed furfural residues. The byproducts(lactic acid, glycerol and acetic acid) concentrations of washed furfural residue in SSF were lower than those of unwashed furfural residue. And the addition of Sapindus saponin could inhibit the produce of byproducts.