|
|||||
|
|
| 两维荧光光谱技术及最佳光谱分辨率设计 | |
| 引用本文： | 刘立新,王磊,屈军乐,林子扬,刘文清,牛憨笨. 两维荧光光谱技术及最佳光谱分辨率设计[J]. 深圳大学学报(理工版), 2007, 24(3): 252-256 |
| 作者姓名： | 刘立新 王磊 屈军乐 林子扬 刘文清 牛憨笨 |
| 作者单位： | 1. 深圳大学光电工程学院,深圳,518060;中国科学院安徽光学精密机械研究所,合肥,230031 2. 深圳大学光电工程学院,深圳,518060 3. 中国科学院安徽光学精密机械研究所,合肥,230031 |
| 基金项目： | 国家自然科学基金;深圳大学校科研和教改项目 |
| 摘 要： | 研究和发展了一种两维荧光光谱测量及成像技术,讨论获得两维空间离散点的最佳光谱分辨率的方法.该技术以棱镜色散原理为基础,利用微透镜阵列实现对样品的离散化照明,充分发挥光学系统固有的并行处理能力,实现了在一个面阵探测器上同时记录两维空间的荧光光谱图像.与逐点扫描方法相比,该技术的扫描效率随激发点数线性提高,具有快速测量、高空间和高光谱分辨的特点,可用于生物医学领域. |
| 关 键 词： | 荧光光谱技术 光谱分辨率 棱镜 微透镜阵列 生物医学 |
| 文章编号： | 1000-2618（2007）03-0252-05 |
| 修稿时间： | 2007-04-17 |
Technique of two-dimensional fluorescence spectroscopy and design for optimal spectral resolution |
|
| LIU Li-xin,WANG Lei,QU Jun-le,LIN Zi-yang,LIU Wen-qing,NIU Han-ben. Technique of two-dimensional fluorescence spectroscopy and design for optimal spectral resolution[J]. Journal of Shenzhen University(Science &engineering), 2007, 24(3): 252-256 | |
| Authors: | LIU Li-xin WANG Lei QU Jun-le LIN Zi-yang LIU Wen-qing NIU Han-ben |
| Affiliation: | 1.College of Optoelectronic Engineering Shenzhen University Shenzhen 518060 P. R. China;2. Anhui Institute of Optics and Fine Mechanics Chinese Academy of Sciences Anhui 230031 P. R. China |
| Abstract: | A 2D fluorescence spectroscopy technique was proposed,and a design method for optimal spectral resolution of 2D discrete points was discussed.This new technique was based on prism dispersion theory.It emploied a microlens array for discrete illumination on the samples.With the intrinsically parallel processing capability of the optical elements and system,the 2D spectrally resolved images can be recorded with one array detector at the same time.Compared with the pixel-by-pixel laser scanning technique,the scanning efficiency of this technique is improved in linear proportion to the excitation spots.Due to its capability of quick measurement and high spatial and spectral resolutions,this technique is expected to have wide applications in biomedicine field. |
| Keywords: | fluorescence spectroscopy technique spectral resolution prism microlens array biomedicine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |