| 液相色谱-电喷雾离子阱质谱分析乌头碱及其代谢物 |
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| 引用本文： | 陈学国,赖永权,蔡宗苇. 液相色谱-电喷雾离子阱质谱分析乌头碱及其代谢物[J]. 质谱学报, 2012, 33(2): 65-73 |
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| 作者姓名： | 陈学国 赖永权 蔡宗苇 |
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| 作者单位： | 1. 中国刑事警察学院法医系，辽宁 沈阳110854; 2. 香港浸会大学化学系，香港特别行政区999077 |
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| 基金项目： | 香港浸会大学项目(FRG/08-09/II-01)资助 |
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| 摘 要： | 为了研究乌头碱的生物转化规律,建立了乌头碱及其代谢物分离与鉴定的液相色谱-电喷雾离子阱质谱(LC-ESI-ITMS)方法。首先利用体外代谢法得到大鼠肝微粒体S9组分中乌头碱及代谢产物混合体系,然后利用LC-ESI-ITMS联用技术对各代谢物进行分析,得到乌头碱代谢物的[M+H]+分子质量信息,进一步结合多级串联质谱(LC/MSn)分析结果获得结构信息,对代谢物进行鉴定,从而推测代谢物结构,并结合大鼠尿中乌头碱及其代谢物的分析结果,得到乌头碱的生物转化规律。本方法在大鼠肝微粒体S9组分中鉴定得到8种体外代谢产物。
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| 关 键 词： | 液相色谱-电喷雾离子阱质谱 肝微粒体 乌头碱 代谢 |
Simultaneous Analysis of Aconitine and Its Metabolites by Liquid Chromatography-Electrospray Ion Trap Mass Spectrometry |
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| CHEN Xue-guo,LAI Yong-quan,CAI Zong-wei. Simultaneous Analysis of Aconitine and Its Metabolites by Liquid Chromatography-Electrospray Ion Trap Mass Spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2012, 33(2): 65-73 |
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| Authors: | CHEN Xue-guo LAI Yong-quan CAI Zong-wei |
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| Affiliation: | 1. Department of Forensic Medicine, China Criminal Police University, Shenyang 110854, China; 2. Department of Chemistry, Hong Kong Baptist University, Hong Kong SAR 999077, China |
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| Abstract: | A method applying liquid chromatography-electrospray ionization-ion trap mass spectrometry(LC-ESI-ITMS) for the simultaneous analysis of Aconitine and its in-vitro metabolites is described.Aconitine was incubated with mouse liver microsome S9 fraction firstly,and then the incubation sample was analyzed by LC-ESI-ITMS in full-scan mode.Aconitine and its metabolites were identified from the determination of molecular ions.The metabolites detection were confirmed from tandem mass spectrometry(MSn) analysis.Comparing with the analysis of urine,8 metabolites were identified in the mouse liver S9 fractions by comparing with the analysis of urine of rat oral with Aconitine and referring to the literature reports. |
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| Keywords: | liquid chromatography-electrospray ion trap mass spectrometry mouse liver S9 fractions Aconitine in-vitro metabolism |
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