HPLC检测功能食品中羟基柠檬酸主成份和两种非法添加药物

目的 利用高效液相色谱(HPLC)建立羟基柠檬酸(Hydroxycitric acid, HCA)功效成分的分析方法, 并建立功能食品中非法添加利莫那班(Rimonabant)和西布曲明(Sibutramine)的同时检测方法。方法 样品经过超声提取, 分别以99 % 0.1 %磷酸水溶液?1 %甲醇为流动相, 在210 nm检测波长下进行HCA检测; 以100 %甲醇为流动相, 在223 nm波长下进行利莫那班和西布曲明同时检测。 结果 HCA在0.001~1.0 mg/mL范围内线性关系良好, 平均添加回收率为90.49 %~103.90 %。利莫那班和西布曲明在0.01~20??g/mL范围内线性关系良好, 检出限分别达到1 ng/mL和3 ng/mL。结论 该方法简单、分析速度快、准确度高, 能够定量检测功能食品中羟基柠檬酸含量, 以及非法添加利莫那班和西布曲明, 实际用于该类功能食品的质量监控效果良好。

HPLC analysis of the active component and two illegally added compounds in hydroxycitric acid functional food

Objective To established an HPLC method for the analysis of hydroxycitric acid (HCA) and the simultaneous determination of illegally added rimonabant and sibutramine in functional food. Methods After ultrasonic extraction of the sample, HCA was analyzed at 210 nm wave length using 99% phosphoric acid (0.1%) ?1% methanol as the mobile phase; Rimonabant and sibutramine was detected at 223 nm wave length using 100% methanol as the mobile phase. Results HCA showed a good liner range between 0.001~1.0 mg/mL with an average spiked recovery of 90.49% ~103.90%; Rimonabant and sibutramine detection reached a liner range between 0.01~20 ?g/mL, and the limit of detection (LOD) was 1 ng/mL and 3 ng/mL, respectively. Conclusion The analysis of HCA and detection of rimonabant and sibutramine were simply operated with high analysis speed and high accuracy which was proved to be practical when used for quality monitoring of functional foods.