涡旋辅助离子液体双水相萃取五味子中木脂素类化合物

建立一种简单、高效、绿色的涡旋辅助离子液体双水相法萃取五味子中7种木脂素类化合物,结合高效液相色谱法对目标物进行分离和定量.研究以离子液体[C4mim]BF4为萃取剂,以MgSO4为成相盐,在涡旋辅助作用下形成双水相体系且目标物则被萃取至离子液体相中,分别对双水相体系的组成和萃取条件进行了考察和优化,并进行了方法评价....

Vortex-assisted Ionic Liquid Aqueous Two-phase Extraction of Lignans from Schisandra chinensis

A vortex-assisted ionic liquid aqueous two-phase extraction coupled with high performance liquid chromatography (HPLC) method was developed and applied to the determination of seven lignans in Schisandra chinensis. In this study, [C₄mim]BF₄ and MgSO₄ were used as the extraction solvent and phase forming salt, respectively. With the aid of vortex (1.5 min), the target analytes were extracted into the ionic liquid phase. Then, the composition of the aqueous two-phase system and the extraction conditions were investigated. The results showed that the best extraction yield of lignans in Schisandra chinensis was achieved when the volume of [C₄mim]BF₄ was 500 μL, the amount of MgSO₄ was 1.6 g and centrifugation condition was at 3000 r/min for 10 min. All target compounds showed a good linear relationship (r>0.9995) in the linear range. The limits of detection and quantification were 0.20~6.98 and 0.65~23.26 ng·mL?1, respectively. The intra-day and inter-day precision (RSD) were lower than 3.97% and 4.19%, respectively. The spiked sample recoveries ranged from 72.22% to 97.19%. Compared with the traditional extraction methods, the extraction time and solvent consumption of this method were reduced. It had the advantages of simple operation, time saving, high efficiency and green, which could be used for the extraction and detection of lignans.