| 高效液相色谱-高分辨率质谱联用技术鉴定阿胶块中不同胶类的特征性多肽 |
| |
| 引用本文： | 沙小梅,张丽君,蒋文丽,王光耀,谢作桦,黄国太,涂宗财. 高效液相色谱-高分辨率质谱联用技术鉴定阿胶块中不同胶类的特征性多肽[J]. 食品工业科技, 2022, 43(4): 97-104. DOI: 10.13386/j.issn1002-0306.2021060180 |
| |
| 作者姓名： | 沙小梅 张丽君 蒋文丽 王光耀 谢作桦 黄国太 涂宗财 |
| |
| 作者单位： | 1.江西师范大学生命科学学院，国家淡水鱼加工技术研发专业中心，江西南昌 3300222.江西德上制药股份有限公司，江西樟树 3312083.江西德上医药研究院有限公司，江西樟树 3312084.南昌大学，食品科学与技术国家重点实验室，江西南昌 330047 |
| |
| 基金项目： | 国家自然科学基金项目(31860428,32160576);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-45)。 |
| |
| 摘 要： | 为研究阿胶块中不同明胶的来源,本文以4种品牌的阿胶块为原料,向其中分别加入低含量(1 mg/mL)的牛明胶和猪明胶,采用高效液相色谱-质谱联用技术(high-performance liquid chromatography-mass spectrometry,HPLC-MS/MS)分别鉴定阿胶、牛明胶和猪明胶的特征性...
|
| 关 键 词： | 阿胶 高效液相色谱-质谱联用技术 溯源鉴定 牛明胶 猪明胶 |
| 收稿时间： | 2021-06-21 |
Identification of Characteristic Peptides of Different Gelatins in Donkey-hide Gelatin Lump by High Performance Liquid Chromatography-High Resolution Mass Spectrometry |
| |
| SHA Xiaomei,ZHANG Lijun,JIANG Wenli,WANG Guangyao,XIE Zuohua,HUANG Guotai,TU Zongcai. Identification of Characteristic Peptides of Different Gelatins in Donkey-hide Gelatin Lump by High Performance Liquid Chromatography-High Resolution Mass Spectrometry[J]. Science and Technology of Food Industry, 2022, 43(4): 97-104. DOI: 10.13386/j.issn1002-0306.2021060180 |
| |
| Authors: | SHA Xiaomei ZHANG Lijun JIANG Wenli WANG Guangyao XIE Zuohua HUANG Guotai TU Zongcai |
| |
| Affiliation: | 1.National R & D Center for Freshwater Fish Processing, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China2.Jiangxi Deshang Pharmaceutical Co., Ltd., Zhangshu 331208, China3.Jiangxi Deshang Pharmaceutical Research Institute Co., Ltd., Zhangshu 331208, China4.State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China |
| |
| Abstract: | To study the source of different gelatins of donkey-hide gelatin lumps,four kinds of donkey-hide gelatin lumps were added with low content(1 mg/mL)of bovine gelatin and porcine gelatin respectively.High-performance liquid chromatography-mass spectrometry(HPLC-MS/MS)were chosen to identify the characteristic peptides of donkey-hide gelatin,bovine gelatin and porcine gelatin.The results showed that 18,3 and 5 common characteristic peptides were accurately detected in the four kinds of donkey-hide gelatin lump for donkey-hide gelatin,bovine gelatin and porcine gelatin,respectively,with 10,1 and 1 from theα1 chain and 8,2 and 4 from theα2 chain.Meanwhile,the number of common characteristic peptides with hydroxylated modifications was 15,2 and 4 respectively,while those without any modification groups were 3,1 and 1.These common characteristic peptides would provide a basis for the HPLC-MS/MS traceable identification of the gelatin source in donkey-hide gelatin lump,which is of reference significance for the quality assurance of donkey-hide gelatin lump products. |
| |
| Keywords: | donkey-hide gelatin high-performance liquid chromatography-mass spectrometry traceability identification bovine gelatin porcine gelatin |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《食品工业科技》浏览原始摘要信息 |
|
点击此处可从《食品工业科技》下载全文 |
|
