人参、甘草、五味子复合醋提物对老年小鼠急性炎性肝损伤保护作用的研究

目的:本研究旨在通过脂多糖(Lipopolysaccharide,LPS)诱导肝损伤小鼠考察复合醋提物(MVE)对老年小鼠炎性肝损伤的保护效果.方法:将小鼠随机分为正常对照组、MVE组、LPS组、LPS+MVE组.通过苏木精-伊红染色评估肝组织病理情况和免疫组织化学染色判断髓过氧化物酶的表达变化.同时,检测肝组织中白介...

Protective Effect of Medicine Vinegar Extract of Panax ginseng,Glycyrrhiza and Schisandra chinensis on Acute Liver Injury in Mice

Objective:Investigating the protective effect of MVE was the purpose of this study,which performed on LPSinduced liver injury in aged mice.Methods:The mice were randomized into the groups of normal control,MVE,LPS and LPS+MVE.The pathological changes of liver tissue and the expression of myeloperoxidase were observed by hematoxylineosin staining and immunohistochemical staining.The levels of IL-1β,IL-17,CXCL1,CXCL17 and other factors in liver were measured.Western blotting was used to detect the expressions of TLR4 and NF-κB p65 in liver tissue samples.Results:The MVE could effectively decline the infiltration of neutrophils and inflammatory cells,significantly decrease the levels of ALT,AST,ROS(P<0.01),pro-inflammatory cytokines and neutrophil chemokines(P<0.05)in the liver of LPS model mice,and decrease protein expressions of TLR4 and NF-κB p65 in inflammatory pathway(P<0.001).Conclusion:The MVE could improve the liver injury of mice with acute inflammation induced by LPS,and exert its hepatoprotective mechanism by regulating the balance of pro-inflammatory and anti-inflammatory factors and inhibiting TLR4-NF-κB signal pathway.