基于IKnife-REIMS技术对地理标志保护产品习水麻羊产地溯源的研究

本研究建立了一种采用快速蒸发电离质谱技术(REIMS)连接高分辨质谱快速鉴定习水麻羊的方法.通过对不同产地羊肉样品的实地收集、仪器采集参数优化(电压设定为40V,长度2 cm,切口深度0.5 cm,切割时间2～3 s)和模型建立,得到一个实时溯源习水麻羊的检测方法.用主成分分析、偏最小二乘法判别分析建立模型,选取变量权...

Research on the Origin Traceability of Geographical Indication Protection Products Xishui Brown Goat Based on IKnife-REIMS Technology

In the reserch,a method for rapid identification of Xishui brown goat by rapid evaporative ionization mass spectrometry(REIMS)was established.This study obtained a real-time traceability detection method for Xishui brown goat conducting optimization of instrument parameters(The voltage was set to 40 V,the length was 2 cm,the incision depth was 0.5 cm,and the cutting time was 2 to 3 s)and model building after collection of lamb samples from different origins.Models were established using principal component analysis and partial least squares discriminant analysis,and marker compounds with variable weight value(VIP)>1 and ANOVA non-parametric test P value≤0.05 were selected to establish identification component score plots,Loadings plots and abundance plots to identify the characteristic markers with large differences.The results showed that the model had good clustering separation effect and could be distinguished from other groupings with the identification accuracy of 97.32%.A number of characterized compounds with large difference were identified by LIPID MAPS database including sphingosine,phosphatidylinositol,heptadecadienoic acid,eicosadienoic acid and succinic acid.The developed testing method would provide a new identification idea for the origin traceability of Xishui brown goat with various advantages of pre-treatment free,short detection time,high recognition rate and stability.