刺玫果中花色苷提取工艺优化及抗氧化性分析

为了优化刺玫果中花色苷提取工艺并考察其抗氧化活性,本研究采用超声波辅助提取法从刺玫果中提取花色苷,通过单因素实验、Placket-Burman设计并结合Box-Behnken实验确定刺玫果中花色苷最佳提取条件;采用清除DPPH·的能力、超氧阴离子自由基(O2-·)能力、ABTS+.能力等多种体外抗氧化方法评价刺玫果中花...

Optimization of Extraction Technique and Antioxidant Activity of Anthocyanins from Rosa davurica Pall.

In order to optimize the extraction conditions of anthocyanins from Rosa davurica Pall.and investigate its antioxidant activity,single-factor experiment,Placket-Bumrman design,Box-Behnken analysis associated with ultrasonic extraction method were adopted.The antioxidant activity of anthocyanins from Rosa davurica Pall.were investigated by various methods,such as removal DPPH radical,O₂^-,radical,ABTS+racial.The results showed that the optimum extraction conditions were as follows:Solid-liquid ratio was 1:15 g·mL^?1,ultrasonic temperature was 50℃,ethanol volume was 60%,ultrasonic time was 30 min,pH was 2,and extraction times was 2.The content anthocyanins of Rosa davurica Pall.was 185.033 mg·100 g^?1.The antioxidant activity was measured with IC₅₀ and the results showed that the removal ability to DPPH free radical,superoxide anion free radical,ABTS+free racial of the anthocyanins was 2.299,1.902,8.993 mg·mL^?1,respectively.The technology in the current study was easy and feasible.The content of anthocyanins was considerable.Furthermore,it showed that the anthocyanins had certain antioxidant activity in various antioxidant experiments.