Host Cell Mimic Polymersomes for Rapid Detection of Highly Pathogenic Influenza Virus via a Viral Fusion and Cell Entry Mechanism |
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| Authors: | Woonsung Na Minjoo Yeom Jihye Choi Jihye Kim Jong‐Woo Lim Dayeon Yun Haejin Chun Geunseon Park Chaewon Park Jeong‐Ki Kim Dae Gwin Jeong Van Phan Le Kwangyeol Lee Jae Myun Lee Hyoung Hwa Jeong Daesub Song Seungjoo Haam |
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| Affiliation: | 1. Department of Pharmacy, College of Pharmacy, Korea University, Sejong, Republic of Korea;2. Research Institute of Radiological Science, College of Medicine, Yonsei University, Seoul, Republic of Korea;3. Department of Chemical & Biomolecular Engineering, Yonsei University, Seoul, Republic of Korea;4. Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea;5. Department of Microbiology and Infectious Diseases, College of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Vietnam;6. Department of Chemistry, Korea University, Seoul, Republic of Korea;7. Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science (IBS), Seoul, Republic of Korea;8. Department of Microbiology, Yonsei University College of Medicine, Seoul, Republic of Korea;9. HuVet bio, Inc., Seoul, Republic of Korea |
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| Abstract: | Highly pathogenic avian influenza virus (HPAIV) infections have occurred continuously and crossed the species barrier to humans, leading to fatalities. A polymerase chain reaction based molecular test is currently the most sensitive diagnostic tool for HPAIV; however, the results must be analyzed in centralized diagnosis systems by a trained individual. This requirement leads to delays in quarantine and isolation. To control the spread of HPAIV, rapid and accurate diagnostics suitable for field testing are needed, and the tests must facilitate a differential diagnosis between HPAIV and low pathogenic avian influenza virus (LPAIV), which undergo cleavage specifically by trypsin‐ or furin‐like proteases, respectively. In this study, a differential avian influenza virus rapid test kit is developed and evaluated in vitro and using clinical specimens from HPAIV H5N1‐infected animals. It is demonstrated that this rapid test kit provides highly sensitive and specific detection of HPAIV and LPAIV and is thus a useful field diagnostic tool for H5N1 HPAIV outbreaks and for rapid quarantine control of the disease. |
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| Keywords: | cell mimics highly pathogenic avian influenza virus (HPAIV) influenza A virus point‐of‐care testing (POCT) polymersomes |
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